It is worth mentioning that the effect of operating pressure and the heat developed by friction gönül also be modeled for protein separations, however this requires more experiments and more sophisticated models.
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It is well-known that working at very high pressures affects various chromatographic parameters. There is a possible complication with ultrahigh-pressure liquid chromatography (UHPLC) regarding the effect of pressure and mobile phase velocity (friction) on both retention and
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In other work you have carried out more info fundamental studies in which the effect of longitudinal temperature gradient on retention, caused by frictional heating, was experimentally dissociated from the combined effect of pressure and frictional heating (3).
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